一种新型无钩豆状囊尾蚴的鉴定和18S DNA序列分析

doi:10.11843/j.issn.0366-6964.2019.11.012

Abstract

Abstract: This study was designed to identify the difference between no-hook Cysticercus pisiformis (C. pisiformis) and hook C. pisiformis. Scolex's ultrastructures of C. pisiformis were observed by scanning electron microscope; PCR amplification and sequences analysis of 18S DNA of C. pisiformis were conducted and evolutionary tree comparison was performed among C. pisiformis and other animals' cysticercus in this study. The results showed that different ultrastructures of no-hook and hook C. pisiformis were mainly observed on rostellum, but the ultrastructures of suck and somite were alike. Most of the C. pisiformis taken out from the vesicles were in relative rest state. The rostellum of no-hook C. pisiformis looked like several oblate pastries stacked up together. Its surface was flatter and had a bigger nodule in the center, in which the diameter was about 20 μm. There were several layers of gear like patterns around the rostellum,forming an overlapping gear like structure. The front end of rostellum of hook C. pisiformis was blunt, and was connected to the chicken claw like hamula through the tegument muscular columns. The hamula were about 120-150 μm long, and its surface was smooth, rich luster and possessed hard appearance. Using 18S-P1 and 18S-P2 primers, the same target gene bands were obtained by PCR in the target genes of no-hook and hook C. pisiformis, culture medium of competent cells and bacterial plasmid. The sequencing results showed that the difference of the 750 bp sequences amplified by 18S-P1 primers was more significant than that of the 666 bp sequences amplified by 18S-P2 primers. Compared with evolution of Cysticercus of other animals, the no-hook C. pisiformis was related to C. saginate, while the hook C. pisiformis was related to C. solium. In conclusion, although both no-hook and hook C. pisiformis belong to the same genus, no-hook C. pisiformis is a new C. pisifomis being discovered.

CLC Number:

S852.734

PAN Yaoqian, KONG Lingyun, LI Peng, YUE Feng, WU Yuping, LIU Xingyou. Identification and 18S DNA Sequence Analysis of a New Type No-hook Cysticercus pisiformis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2283-2289.

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References 22

[1]	KYNGDON C T, GAUCI C G, ROLFE R A, et al. In vitro oncosphere-killing assays to determine immunity to the larvae of Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium[J]. J Parasitol, 2006, 92(2):273-281.
[2]	SZKUCIK K, PYZ-LUKASIK R, SZCZEPANIAK K O, et al. Occurrence of gastrointestinal parasites in slaughter rabbits[J]. Parasitol Res, 2014, 113(1):59-64.
[3]	STANCAMPIANO L, RAVAGNAN S, CAPELLI G, et al. Cysticercosis by Taenia pisiformis in Brown Hare (Lepus europaeus) in Northern Italy:epidemiologic and pathologic features[J]. Int J Parasitol Parasites Wildl, 2019, 9:139-143.
[4]	冉靖北, 许光阳,沈会川. 家兔豆状囊尾蚴病暴发感染调查[J]. 中国养兔杂志, 2000(2):11-13.
	RAN J B, XU G Y, SHEN H C. Survey of outbreak infection of Cysticercosis pisiformis in rabbit[J]. Chinese Journal of Rabbit Farming, 2000(2):11-13. (in Chinese)
[5]	杨德英, 杨光友. 兔豆状囊尾蚴病研究进展[J]. 中国畜牧兽医, 2015, 42(4):1015-1020.
	YANG D E, YANG G Y. Research progress on rabbit cysticercosis[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(4):1015-1020. (in Chinese)
[6]	赵恒章, 刘保国. 肉兔豆状囊尾蚴病的诊治[J]. 中国草食动物, 2005, 25(1):40-41.
	ZHAO H Z, LIU B G. Diagnosis and treatment for Cysticercus pisiformis of meat rabbit[J]. China Herbivores, 2005, 25(1):40-41. (in Chinese)
[7]	张念,赵振升,王帅,等. 兔豆状囊尾蚴的体外培养研究[J]. 动物医学进展, 2010, 31(4):34-37.
	ZHANG N, ZHAO Z S, WANG S, et al. Study on cultivation of Cysticercus pisiformis in vitro[J]. Progress in Veterinary Medicine, 2010, 31(4):34-37. (in Chinese)
[8]	王帅,韩利方,张高奎,等. 屠宰兔感染豆状囊尾蚴的体内分布及卫生评价[J]. 动物医学进展, 2009, 30(9):11-15.
	WANG S, HAN L F, ZHANG G K, et al. Distribution of cysts and hygienic evalution of meat quality in slaughtered rabbits infected with Cysticercus pisiformis[J]. Progress in Veterinary Medicine, 2009, 30(9):11-15. (in Chinese)
[9]	鲁恩荣, 孙健,王莹,等. 医学实验家兔感染豆状囊尾蚴病的初步调查[J]. 微量元素与健康研究, 2016, 33(3):84.
	LU E R, SUN J, WANG Y, et al. A preliminary investigation of Medical experimental rabbits infected with Cysticercosis pisiformis[J]. Studies of Trace Elements and Health, 2016, 33(3):84. (in Chinese)
[10]	刘巧霞. 兔豆状囊尾蚴病的临床症状及防治措施[J]. 中国养兔, 2016(5):28-29.
	LIU Q X. Clinical symptoms and controlling measure of Cysticercosis pisiformis in rabbit[J]. Chinese Journal of Rabbit Farming, 2016(5):28-29. (in Chinese)
[11]	曹民川, 周丽梅,唐振兴. 兔豆状囊尾蚴病防治技术[J]. 贵州畜牧兽医, 2017, 41(6):46-47.
	CAO M C, ZHOU L M, TANG Z X. Prevention and treatment of rabbit cysticercosis[J]. Guizhou Journal of Animal Husbandry & Veterinary Medicine, 2017, 41(6):46-47. (in Chinese)
[12]	潘耀谦, 刘兴友,赵振升,等. 兔豆状囊尾蚴的压片技术及染色方法研究[J]. 动物医学进展, 2009, 30(11):21-24.
	PAN Y Q, LIU X Y, ZHAO Z S, et al. Study on squash technique and staining methods for scolex of Cysticercus pisiformis[J]. Progress in Veterinary Medicine, 2009, 30(11):21-24. (in Chinese)
[13]	YANG D Y, CHEN L, WU X H, et al. Expression of the Tpanxb1 gene from Taenia pisiformis and its potential diagnostic value by dot-ELISA[J]. J Parasitol, 2014, 100(2):246-250.
[14]	潘耀谦, 冯春花,王帅,等. 诊断兔豆状囊尾蚴病的阻断ELISA方法的建立和初步应用[J]. 中国兽医学报, 2018, 38(9):1704-1710.
	PAN Y Q, FENG C H, WANG S, et al. Establishment and preliminary application of competitive ELISA for diagnosis of Cysticercosis pisiformis in rabbit[J]. Chinese Journal of Veterinary Science, 2018, 38(9):1704-1710. (in Chinese)
[15]	PAN Y Q, ZHANG L P, WANG S, et al. Ultrastructural changes of scolex on Cysticercus pisiformis during relative rest and motion states[J]. Agric Sci Technol, 2014, 15(1):111-115.
[16]	潘耀谦, 李瑞珍,银梅,等. 有钩与无钩豆状囊尾蚴头节超微形态的比较[J]. 畜牧兽医学报, 2016, 47(12):2493-2500.
	PAN Y Q, LI R Z, YIN M, et al. Ultra-morphological comparative observation on scolex of hook and no-hook Cysticercus pisiformis[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(12):2493-2500. (in Chinese)
[17]	潘耀谦, 刘兴友,赵振升,等. 豆状囊尾蚴的超微结构观察[J]. 畜牧兽医学报, 2012, 43(4):653-658.
	PAN Y Q, LIU X Y, ZHAO Z S, et al. Ultrastructure observation of Cysticercus pisiformis[J]. Acta Veterinaria et Zootechnica Sinica, 2012, 43(4):653-658. (in Chinese)
[18]	汪明. 兽医寄生虫学[M]. 3版. 北京:中国农业出版社, 2003.
	WANG M. Veterinary parasitology[M]. 3rd ed. Beijing:China Agriculture Press, 2003. (in Chinese)
[19]	张西臣, 李建华. 动物寄生虫病学[M]. 3版. 北京:科学出版社, 2010.
	ZHANG X C, LI J H. Veterinary parasitology[M]. 3rd ed. Beijing:Science Press, 2010. (in Chinese)
[20]	孔繁瑶. 家畜寄生虫学[M]. 北京:中国农业大学出版社, 2008.
	KONG F Y. Livestock parasitology[M]. Bejing:China Agriculture University Press, 2008. (in Chinese)
[21]	潘耀谦, 唐海蓉,冯春花,等. 一种新的豆状囊尾蚴——无钩豆状囊尾蚴的超微结构观察[J]. 中国兽医学报, 2017, 37(9):1710-1714.
	PAN Y Q, TANG H R, FENG C H, et al. A new Cysticercus pisiformis:ultrastructural observation of no-hamulus Cystcercus pisiformis[J]. Chinese Journal of Veterinary Science, 2017, 37(9):1710-1714. (in Chinese)
[22]	孙晓林, 骆学农,王晓霞,等. 兔豆状囊尾蚴和豆状带绦虫头节的组织结构观察[J]. 中国兽医科学, 2008, 38(9):796-800.
	SUN X L, LUO X N, WANG X X, et al. Observation of the organizational structure of Cysticercus pisiformis and Taenia pisiformis scolex[J]. Chinese Veterinary Science, 2008, 38(9):796-800. (in Chinese)

Identification and 18S DNA Sequence Analysis of a New Type No-hook Cysticercus pisiformis

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